Transmembrane Delivery of the Cell-Penetrating Peptide Conjugated Semiconductor Quantum Dots

被引:83
作者
Chen, Bo [1 ,2 ]
Liu, Qiaoling [1 ,2 ]
Zhang, Yuliang [1 ]
Xu, Li [1 ]
Fang, Xiaohong [1 ]
机构
[1] Chinese Acad Sci, Inst Chem, BNLMS, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
基金
中国国家自然科学基金;
关键词
D O I
10.1021/la802048s
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Conjugation of the cell-penetrating peptide derived from the human immunodeficiency virus-1 transactivator protein (TAT) to semiconductor quantum dots (QDs) is an effective way to enhance transmembrane delivery of QDs for intracellular and molecular imaging. In this work, the internalization pathway of TAT-QDs was studied systematically in living cells. Cellular uptake of TAT-QDs, under different endocytosis-inhibiting conditions, was compared by fluorescence imaging and flow cytometry. The results suggest TAT-QDs internalize through lipid-raft-dependent macropinocytosis, which is different from that of FITC-labeled TAT. They also provide new information for better understanding of the TAT-mediated cell uptake mechanism.
引用
收藏
页码:11866 / 11871
页数:6
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