Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis

被引：59

作者：

Carroll, BJ ^{[1
]}

Moss, SJ ^{[1
]}

Bai, LQ ^{[1
]}

Kato, Y ^{[1
]}

Toelzer, S ^{[1
]}

Yu, TW ^{[1
]}

Floss, HG ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 16期

关键词：

D O I：

10.1021/ja0124764

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The unusual "glycolate" extender unit at C-9/C-10 of ansamitocin is not derived from 2-hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-13C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin bisosynthetic gene cluster. Copyright © 2002 American Chemical Society.

引用

页码：4176 / 4177

页数：2

共 22 条

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