Culture of Helicobacter pylori requires micro-aerobic conditions which are usually generated by a commercial gas-generator system. However, the commercial gas-generator pack is not available in most hospitals in developing countries, The present study showed that H. pylori grew well in a candle jar system that provided microaerobic condition; this gave similar cultural results to a commercial gas-generator system. Therefore, the candle jar system can be used for H. pylori micro-aerobic culture in developing countries where commercial systems are not available.