Carbohydrate analysis of bradyrhizobial (NC 92) lipopolysaccharides by high performance-anion exchange chromatography with pulsed amperometric detection

被引：9

作者：

Das, H ^{[1
]}

Jayaraman, V ^{[1
]}

Bhattacharya, I ^{[1
]}

机构：

[1] Ctr Biochem Technol, Delhi 110007, India

来源：

BIOSCIENCE REPORTS | 1999年 / 19卷 / 03期

关键词：

Bradyrhizobia; HPAE-PAD; legume lectin; lipopolysaccharide;

D O I：

10.1023/A:1020281921029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Composition analysis of monosaccharides of Sepharose 4B purified NC 92 LPS and the polysaccharides fractions from Sephadex G-50 chromatography was performed by high performance anion exchange chromatography using pulsed amperometric detection. Rhamnose, mannose, galactose and glucose are present in a substantial amount in the purified LPS (Pk I). High molecular weight purified polysaccharides (PS I) obtained after sephadex gel filtration of the purified LPS (Pk I) acid hydrolysate showed an increase in glucose:galactose ratio. This indicates the presence of the peanut root lectin (PRA II) specific sugar in higher proportion on the O-antigen part of the LPS molecule, which may aid in the critical recognition reaction.

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页码：219 / 225

页数：7

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