A high-pressure liquid chromatographic-tandem mass spectrometric method for the determination of ethambutol in human plasma, bronchoalveolar lavage fluid, and alveolar cells

被引：25

作者：

Conte, JE ^{[1
]}

Lin, E ^{[1
]}

Zhao, YP ^{[1
]}

Zurlinden, E ^{[1
]}

机构：

[1] Univ San Francisco, Dept Epidemiol & Biostat, Infect Dis Res Lab, San Francisco, CA 94117 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2002年 / 40卷 / 02期

关键词：

D O I：

10.1093/chromsci/40.2.113

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique is presented for the specific and sensitive determination of ethambutol concentrations in plasma, bronchoalveolar lavage (BAL), and alveolar cells (AC) using a high-pressure liquid chromatographic (HPLC)-tandem mass spectrometric (MS-MS) method. The preparation of samples requires a deproteinization step with acetonitrile. The retention times for ethambutol, neostigmine bromide, and propranolol are 2.0, 1.4, and 1.1 min, respectively, with a total run time of 2.8 min. The detection limits for ethambutol are 0.05 μg/mL for plasma and 0.005 μg/mL for the BAL supernatants and AC suspensions. The assay has excellent performance characteristics and has been used to support a study of the intrapulmonary pharmacokinetics of ethambutol in human subjects.

引用

页码：113 / 118

页数：6

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