The three-dimensional structure of CsmA:: A small antenna protein from the green sulfur bacterium Chlorobium tepidum

被引：33

作者：

Pedersen, Marie Ostergaard ^{[1
,2
]}

Underhaug, Jarl ^{[1
,2
]}

Dittmer, Jens ^{[1
,2
]}

Miller, Mette ^{[3
]}

Nielsen, Niels Chr. ^{[1
,2
]}

机构：

[1] Univ Aarhus, Interdisciplinary Nanosci Ctr iNANO, Ctr Insoluble Prot Structures inSPIN, DK-8000 Aarhus C, Denmark

[2] Univ Aarhus, Dept Chem, DK-8000 Aarhus, Denmark

[3] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark

来源：

FEBS LETTERS | 2008年 / 582卷 / 19期

基金：

新加坡国家研究基金会;

关键词：

chlorosome; CsmA; green bacterium; nuclear magnetic resonance; photosynthetic antenna; Chlorobium tepidum;

D O I：

10.1016/j.febslet.2008.07.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the chlorosome baseplate protein CsmA from Chlorobium tepidum in a 1: 1 chloroform: methanol solution was determined using liquid-state NMR spectroscopy. The data reveal that the 59-residue protein is predominantly alpha-helical with a long helical domain extending from residues V6 to L36, containing a putative bacteriochlorophyll a binding domain, and a short helix in the C-terminal part extending from residues M41 to G49. These elements are compatible with a model of CsmA having the long N-terminal alpha-helical stretch immersed into the lipid monolayer con. ning the chlorosome and the short C-terminal helix protruding outwards, thus available for interaction with the Fenna-Matthews-Olson antenna protein. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2869 / 2874

页数：6

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