Alterations and role of human cathepsin D in cancer metastasis

被引：45

作者：

Rochefort, H

Liaudet, E

Garcia, M

机构：

[1] Inst. Natl. Sante Recherche Medicale, Unit 148, University of Montpellier I, F-33090 Montpellier

来源：

ENZYME & PROTEIN | 1996年 / 49卷 / 1-3期

关键词：

metastasis; breast cancer; proteases; cathepsin D; phagocytosis; growth factor; growth inhibitor; extracellular matrix; bFGF;

D O I：

10.1159/000468620

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cathepsin D (Cath D) overexpression in breast cancer cells is associated with increased risk of metastasis in patients according to several clinical studies. The amino acid sequence of Cath D in two breast cancer cell lines was normal, but glycosylation appears to be different with more acidic isoforms. Transfection of a human cDNA Cath D expression vector increases the metastatic potential of 3Y1-Ad12 embryonic rat tumorigenic cells when intravenously injected into nude mide. The mechanism of Cath-D-induced metastasis seems to require maturation of the proenzyme, mostly in large acidic compartments identified as phagosomes. Cath D is mitogenic in different cell types, and different substrates (growth inhibitors, precursors of growth factors, etc.) are proposed to mediate this activity.

引用

页码：106 / 116

页数：11

共 54 条

[1]

ANGEREAU P, 1988, MOL ENDOCRINOL, V2, P186

[2] CATHEPSIN-D - PURIFICATION OF ISOENZYMES FROM HUMAN AND CHICKEN LIVER [J].