Use of RAPD and 16S rDNA sequencing for the study of Lactobacillus population dynamics in natural whey culture

被引：76

作者：

Cocconcelli, PS ^{[1
]}

Parisi, MG ^{[1
]}

Senini, L ^{[1
]}

Bottazzi, V ^{[1
]}

机构：

[1] UNIV CATTOLICA SACRO CUORE,CTR RIC BIOTECNOL,CREMONA,ITALY

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 25卷 / 01期

关键词：

D O I：

10.1046/j.1472-765X.1997.00061.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The development of communities of the thermophilic microflora of natural whey culture for Parmigiano Reggiano cheese production was studied by means of molecular techniques. RAPD analysis facilitates the identification of the Lactobacillus strains involved in this microbial association and permitted the study of population dynamics during two cycles of whey fermentation. Analysis of RAPD fingerprints revealed the presence of four biotypes that dominate the whey fermentation process. Sequence analysis of 16S rDNA demonstrated that the strains isolated from whey belong to Lact. helveticus and Lact. delbrueckii ssp. lactis species.

引用

页码：8 / 12

页数：5

共 21 条

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