Protein evolution by molecular breeding

被引：84

作者：

Minshull, J ^{[1
]}

Stemmer, WPC ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1999年 / 3卷 / 03期

关键词：

D O I：

10.1016/S1367-5931(99)80044-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural evolution has guided the development of molecular breeding' processes used in the laboratory for the rapid modification of subgenomic sequences including single genes. The most significant recent development has been the in vitro permutation of natural diversity. Homologous recombination of multiple related sequences produced high-quality libraries of chimeric sequences encoding proteins with functions that differ dramatically from any of the parents. Increasingly powerful screening methods are also being developed, allowing these libraries to be screened for novel biocatalysts.

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页码：284 / 290

页数：7

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