Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments

被引:76
作者
Chen, SL
Wilson, DB
机构
[1] CORNELL UNIV, BIOCHEM MOL & CELL BIOL SECT, ITHACA, NY 14853 USA
[2] CORNELL UNIV, INST COMPARAT & ENVIRONM TOXICOL, ITHACA, NY 14853 USA
关键词
D O I
10.1128/AEM.63.6.2442-2445.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg2+-contaminated environments.
引用
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页码:2442 / 2445
页数:4
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