Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments

被引：76

作者：

Chen, SL

Wilson, DB

机构：

[1] CORNELL UNIV, BIOCHEM MOL & CELL BIOL SECT, ITHACA, NY 14853 USA

[2] CORNELL UNIV, INST COMPARAT & ENVIRONM TOXICOL, ITHACA, NY 14853 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 06期

关键词：

D O I：

10.1128/AEM.63.6.2442-2445.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg2+-contaminated environments.

引用

页码：2442 / 2445

页数：4

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