Oxidative damage to nuclear DNA in hyperthyroid rat liver:: Inability of vitamin C to prevent the damage

被引：21

作者：

Andican, G

Gelisgen, R

Civelek, S

Seven, A

Seymen, O

Altug, T

Yigit, G

Burçak, G

机构：

[1] Istanbul Univ, Cerrahpasa Med Fac, Dept Biochem, Istanbul, Turkey

[2] Istanbul Univ, Cerrahpasa Med Fac, Dept Physiol, Istanbul, Turkey

[3] Istanbul Univ, Cerrahpasa Med Fac, Dept DETAM, Istanbul, Turkey

来源：

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | 2004年 / 67卷 / 05期

关键词：

D O I：

10.1080/15287390490273479

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The effects of hyperthyroidism on oxidative DNA damage in liver tissue and modification by vitamin C supplementation were investigated in rats. Animals were rendered hyperthyroid by administration of L-thyroxine (0.4 mg/100g food) for 25 d. In the plasma samples, T-3, T-4, and thyroid-stimulating hormone (TSH) were measured by radioimmunoassay and ascorbate spectrophotometrically. Oxidative damage to hepatic nuclear DNA was determined by measuring deoxy-guanosine (dG) and 8-oxodG by high-performance liquid chromatography with diode array detector electrochemical detection (HPLC-DAD-ECD). In hyperthyroidism, 8-oxodG/(10(5) dG) levels were significantly higher and plasma vitamin C levels lower than in control rats. The results of this experimental study show that oxidative damage to hepatic nuclear DNA increases in the hyperthyroid state and that vitamin C was not effective in preventing this damage.

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页码：413 / 420

页数：8

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