Anticonvulsant and GABA uptake inhibition properties of venom fractions from the spiders Parawixia bistriata and Scaptocosa raptoria

被引：23

作者：

Cairrao, MAR

Ribeiro, AM

Pizzo, AB

Fontana, ACK

Beleboni, RO

Coutinho-Netto, J

Miranda, A

Santos, WF

机构：

[1] Univ Sao Paulo, Lab Neurobiol & Peconhas, Dept Biol, Fac Filosofia,Ciencias & Letras Ribeirao Preto, BR-14049901 Sao Paulo, Brazil

[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Neuroquim, Dept Bioquim, BR-14049901 Sao Paulo, Brazil

[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo, Brazil

来源：

PHARMACEUTICAL BIOLOGY | 2002年 / 40卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

anticonvulsant; bicuculline; GABA; GABA uptake; Parawixia bistriata; pentilenetetrazole; picrotoxin; Scaptocosa raptoria; spider venom; rat synaptosomes;

D O I：

10.1076/phbi.40.6.472.8436

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this article we describe an in vivo anticonvulsant effect from denatured crude venom and partially isolated fractions from two spiders: Parawixia bistriata and Scaptocosa raptoria. Intracerebroventricular injections of these venoms and fractions abolished rat convulsive tonic-clonic seizures induced by picrotoxin, bicuculline and pentylenetetrazole, and also, inhibited GABA uptake in synaptosomes of rat cerebral cortex. The venoms described in this work seems to be promising tools for the study of the GABAergic system, and may be a potential source for new anticonvulsant drugs.

引用

页码：472 / 477

页数：6

共 23 条

[1]

BERGMEYER HV, 1983, METHOD ENZYMAT AN, P343

[2] TIAGABINE, SK-AND-F 89976-A, CI-966, AND NNC-711 ARE SELECTIVE FOR THE CLONED GABA TRANSPORTER GAT-1 [J].

BORDEN, LA ;

DHAR, TGM ;

SMITH, KE ;

WEINSHANK, RL ;

BRANCHEK, TA ;

GLUCHOWSKI, C .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1994, 269 (02) :219-224

[3] SPERMINE AND PHILANTHOTOXIN POTENTIATE EXCITATORY AMINO-ACID RESPONSES OF XENOPUS OOCYTES INJECTED WITH RAT AND CHICK BRAIN-RNA [J].

BRACKLEY, P ;

GOODNOW, R ;

NAKANISHI, K ;

SUDAN, HL ;

USHERWOOD, PNR .

NEUROSCIENCE LETTERS, 1990, 114 (01) :51-56

[4]

BUCHERL W, 1969, AM ZOOL, V9, P157

[5] THE EFFECTS OF SCORPION-VENOM TOXIN ON THE RELEASE OF AMINO-ACID NEUROTRANSMITTERS FROM CEREBRAL-CORTEX INVIVO AND INVITRO [J].

COUTINHONETTO, J ;

ABDULGHANI, AS ;

NORRIS, PJ ;

THOMAS, AJ ;

BRADFORD, HF .

JOURNAL OF NEUROCHEMISTRY, 1980, 35 (03) :558-565

[6] Paralizing activity of the Parawixia bistriata crude venom in termites:: a new bioassay [J].

Fontana, ACK ;

Cairrao, MAR ;

Colusso, AJ ;

Santos, WF ;

Coutinho-Netto, J .

TOXICON, 2000, 38 (01) :133-138

[7]

GRAY EG, 1962, J ANAT, V96, P79

[8] DETERMINATION OF PROTEIN - MODIFICATION OF LOWRY METHOD THAT GIVES A LINEAR PHOTOMETRIC RESPONSE [J].

HARTREE, EF .

ANALYTICAL BIOCHEMISTRY, 1972, 48 (02) :422-&

[9] UPTAKE OF [3H]GABA BY SLICES OF RAT CEREBRAL CORTEX [J].

IVERSEN, LL ;

NEAL, MJ .

JOURNAL OF NEUROCHEMISTRY, 1968, 15 (10) :1141-&

[10] SPIDER TOXINS AS TOOLS FOR DISSECTING ELEMENTS OF EXCITATORY AMINO-ACID TRANSMISSION [J].

JACKSON, H ;

USHERWOOD, PNR .

TRENDS IN NEUROSCIENCES, 1988, 11 (06) :278-283

← 1 2 3 →