Chronic quercetin exposure affects fatty acid catabolism in rat lung

被引:60
作者
de Boer, V. C. J.
van Schothorst, E. M.
Dihal, A. A.
van der Woude, H.
Arts, I. C. W.
Rietjens, I. M. C. M.
Hollman, P. C. H.
Keijer, J.
机构
[1] Univ Wageningen & Res Ctr, RIKILT Inst Food Safety, NL-6700 AE Wageningen, Netherlands
[2] Wageningen Univ, Div Toxicol, Wageningen, Netherlands
[3] TNO Qual Life, Business Univ Biosci, Zeist, Netherlands
关键词
quercetin; microarray; fatty acid metabolism; nutrigenomics; flavonoids; polyphenols;
D O I
10.1007/s00018-006-6316-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by the long-term quercetin intervention. Up-regulation of genes (Hmgcs2, Ech1, Acox1, Pcca, Lpl and Acaa2) was verified and confirmed by quantitative real time PCR. In addition, free fatty acid levels were decreased in rats fed the quercetin diet, confirming that quercetin affects fatty acid catabolism. This in vivo study demonstrates for the first time that fatty acid catabolism is a relevant process that is affected in rats by chronic dietary quercetin.
引用
收藏
页码:2847 / 2858
页数:12
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