Tagging quantitative loci controlling pathogenicity in Magnaporthe grisea by insertional mutagenesis

被引：7

作者：

Fujimoto, D ^{[1
]}

Shi, Y ^{[1
]}

Christian, D ^{[1
]}

Mantanguihan, JB ^{[1
]}

Leung, H ^{[1
]}

机构：

[1] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2002年 / 61卷 / 02期

关键词：

Pyricularia grisea; quantitative trait loci; restriction enzyme mediated integration transformation;

D O I：

10.1006/pmpp.2002.0418

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Quantitative trait loci controlling pathogenicity in the rice blast fungus were tagged by insertional mutagenesis. Seven of 634 (1-1 %) integrative transformants exhibited reduced pathogenicity; cosegregation analysis indicated that three mutants (pat531, pa1144 and pat]94) were tagged by plasmid insertion. Mutants pat144 and pat531 were apparently normal in vegetative growth, sporulation and appressorium formation; however, 10-fold higher inocula (10(6) vs 10(5) spores ml(-1)) were required to achieve wild-type levels of pathogenicity. Reduced pathogenicity was attributed to low infection efficiency and reduced colonization rate of host tissue. Plasmids carrying the inactivated genes were retrieved from the mutants. The identities of the retrieved sequences from pa1144 and pat531 were confirmed by gene replacement and PAT531 was isolated from genomic and cDNA libraries. Complementation with the genomic clone restored the pat531 mutant to the wild-type phenotype. The 1592 bp PAT531 transcript codes for a predicted protein of 369 amino acids. Sequence analyses establish that PAT531 is a transmembrane protein with high similarity to the TB2_DPl_HVA22 family of proteins found in diverse eukaryotes. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：77 / 88

页数：12

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