A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes

被引：200

作者：

Okimoto, Y

Watanabe, A

Niki, E

Yamashita, T

Noguchi, N

机构：

[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Tokyo 1538904, Japan

[2] Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

来源：

FEBS LETTERS | 2000年 / 474卷 / 2-3期

基金：

日本学术振兴会;

关键词：

lipid peroxidation; polymorphonuclear leukocyte; imaging; diphenyl-1-pyrenylphosphine; fluorescent probe;

D O I：

10.1016/S0014-5793(00)01587-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diphenyl-1-pyrrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O), DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor wi:th tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several, kinds of free radicals, It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically, (C) 2000 Federation of European Biochemical Societies.

引用

页码：137 / 140

页数：4

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