Noncoordinate regulation of epithelial Na channel and Na pump subunit mRNAs in kidney and colon by aldosterone

被引:117
作者
Escoubet, B
Coureau, C
Bonvalet, JP
Farman, N
机构
[1] UNIV PARIS 07, FAC MED XAVIER BICHAT, U246, PARIS, FRANCE
[2] UNIV PARIS 07, FAC MED XAVIER BICHAT, DEPT PHYSIOL, PARIS, FRANCE
[3] UNIV PARIS 07, FAC MED XAVIER BICHAT, INSERM, INST FEDERAT RECH CELLULES EPITHELIALES, PARIS, FRANCE
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1997年 / 272卷 / 05期
关键词
sodium transport; gene; rat; sodium pump; subunits;
D O I
10.1152/ajpcell.1997.272.5.C1482
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Distal colon and renal cortical collecting ducts are major effecters of aldosterone-dependent Na homeostasis. Na is absorbed by entry through an apical amiloride-sensitive Na channel and extruded by Na-K-ATPase at the basolateral membrane. Using a ribonuclease protection assay, we studied, in vivo, aldosterone regulation of alpha-, beta-, gamma-subunits of the rat epithelial Na channel (rENaC) and alpha(1)- and beta(1)-subunits of Na-K-ATPase. In the kidney, Na-K-ATPase mRNAs were also assayed over discrete tubular segments by in situ hybridization. In rat colon, all three rENaC mRNAs were decreased by adrenalectomy, with a major effect on beta- and gamma-subunits, and were restored with 7 days, but not 2 days, of aldosterone treatment; in the kidney, however, only a-transcripts varied. Na-K-ATPase alpha(1)- and beta(1)-subunit mRNAs in both organs were not (in the case of the beta(1)-subunit) or were mildly (in the case of the alpha(1)-subunit) affected after adrenalectomy. Our conclusions are as follows: 1) Transcripts of rENaC and Na-K-ATPase subunits are not coordinately regulated by aldosterone in vivo; i.e., modulation involves mainly the Na channel, not Na-K-ATPase; the effect is not of comparable magnitude on each subunit mRNA and differs between tissues. 2) The delay of the aldosterone effect on transcripts is much longer than that required to restore normal Na transport in adrenalectomized rats, indicating that rENaC and Na-K-ATPase subunit transcript levels may depend on unidentified early aldosterone-induced proteins.
引用
收藏
页码:C1482 / C1491
页数:10
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