Expression and function of 5-HT3 receptors in the enteric neurons of mice lacking the serotonin transporter

被引:65
作者
Liu, MT
Rayport, S
Jiang, Y
Murphy, DL
Gershon, MD
机构
[1] Columbia Univ Coll Phys & Surg, Dept Anat & Cell Biol, New York, NY 10032 USA
[2] New York State Psychiat Inst & Hosp, Dept Neurosci, New York, NY 10032 USA
[3] NIMH, Clin Sci Lab, Bethesda, MD 20892 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2002年 / 283卷 / 06期
关键词
serotonin receptors; small intestine; enteric nervous system; electrophysiology;
D O I
10.1152/ajpgi.00203.2002
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The actions of enteric 5-HT are terminated by 5-HT transporter (SERT)-mediated uptake, and gastrointestinal motility is abnormal in SERT -/- mice. We tested the hypothesis that adaptive changes in enteric 5-HT3 receptors help SERT -/- mice survive despite inefficient 5-HT inactivation. Expression of mRNA encoding enteric 5-HT3A subunits was similar in SERT +/+ and -/- mice, but that of 5-HT3B subunits was fourfold less in SERT -/- mice. 5-HT3B mRNA was found, by in situ hybridization, in epithelial cells and enteric neurons. 5-HT evoked a fast inward current in myenteric neurons that was pharmacologically identified as 5-HT3 mediated. The EC50 of the 5-HT response was lower in SERT +/+ (18 muM) than in SERT -/- (36 muM) mice and desensitized rapidly in a greater proportion of SERT -/- neurons; however, peak amplitudes, steady-state current, and decay time constants were not different. Adaptive changes thus occur in the subunit composition of enteric 5-HT3 receptors of SERT -/- mice that are reflected in 5-HT3 receptor affinity and desensitization.
引用
收藏
页码:G1398 / G1411
页数:14
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