Identity tests:: determination of cell line cross-contamination

被引：34

作者：

Cabrera, C. M. ^{[1
]}

Cobo, F. ^{[1
]}

Nieto, A. ^{[1
]}

Cortes, J. L. ^{[1
]}

Montes, R. M. ^{[1
]}

Catalina, P. ^{[1
]}

Concha, A. ^{[1
]}

机构：

[1] Hosp Univ Virgen Nieves, Stem Cell Bank Andalucia Spanish Cent Node, Granada 18014, Spain

来源：

CYTOTECHNOLOGY | 2006年 / 51卷 / 02期

关键词：

cell lines; cross-contamination; fingerprinting; isoenzyme analysis; short tandem repeat; variable number tandem repeat;

D O I：

10.1007/s10616-006-9013-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

引用

页码：45 / 50

页数：6

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