Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria

被引：161

作者：

Tombolini, R

Unge, A

Davey, ME

deBruijn, FJ

Jansson, JK

机构：

[1] UNIV STOCKHOLM, ARRHENIUS LABS NAT SCI, DEPT BIOCHEM, S-10691 STOCKHOLM, SWEDEN

[2] MICHIGAN STATE UNIV, NSF, CTR MICROBIAL ECOL, E LANSING, MI 48824 USA

[3] MICHIGAN STATE UNIV, DEPT MICROBIOL, E LANSING, MI 48824 USA

[4] MICHIGAN STATE UNIV, DOE, PLANT RES LAB, E LANSING, MI 48824 USA

来源：

FEMS MICROBIOLOGY ECOLOGY | 1997年 / 22卷 / 01期

关键词：

flow cytometry; green fluorescent protein (GFP); genetically modified microorganism (GMM); marker gene;

D O I：

10.1016/S0168-6496(96)00068-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene encoding the green fluorescent protein (GFP) from the jellyfish Aequorea victoria was assembled into an expression cassette for bacteria by fusing it to the T7 gene10 ribosome binding site and the strong, constitutive promoter PpsbA from Amaranthus hybridus. By using Tn5-based transposon delivery systems, Pseudomanas fluorescens bacteria were chromosomally tagged with gfp. We demonstrate that expression of a single copy gfp gene is sufficient to permit the visualization of bacteria by epifluorescence and laser confocal microscopy and detection by flow cytometry. The green fluorescent phenotype was detectable in all growth phases even under nutrient-limited conditions.

引用

页码：17 / 28

页数：12

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