Local versus systemic effects of exogenous estradiol-17β on ovarian follicular dynamics in heifers with progestogen implants

Two experiments were designed to determine if the suppressive effect of estradiol treatment on ovarian follicles in progestogen-implanted heifers is mediated directly at the ovary or systemically, at a higher level, The purpose of Experiment 1 was to determine a minimal effective dose of estradiol-17 beta (E-17 beta) that would induce follicle regression in progestogen-implanted heifers. Beef heifers were implanted with progestogen on Day 2. (Day 0 = ovulation) and were assigned randomly to five groups: control (sesame seed oil, n = 9); 0.1 mg of E-17 beta (II = 8); 0.5 mg of E-17 beta (n = 8); 1 mg of E-17 beta in = 8); or 5 mg of E-17 beta (n = 8) by intramuscular (im) injection on Day 3. Treatment with 5 and I mg of E-17 beta resulted in smaller (P < 0.05) day-to-day diameter profiles of the dominant follicle compared with controls, whereas 0.1 mg of E-17 beta did not have an apparent effect on follicle growth. The effect of a dose of 0.5 mg was intermediate and tended (P < 0.06) to result in a smaller diameter profile of the dominant follicle compared with control heifers. Experiment 3 was designed to utilize a subminimal dose of E-17 beta (0.1 mg), locally, to determine whether estradiol treatment induces follicle regression through a direct action on the ovary. Beef heifers received a progestogen ear implant on Day 2 and were assigned randomly to five groups on Day 3: control (sesame seed oil, n = 8); 5 mg of E-17 beta im ((n = 8); 0.1 mg of E-17 beta im (n = 8); 0.1 mg of E-17 beta given into the wall of the uterus, near the tip of the horn ipsilateral to the dominant follicle (intrauterine (iu), II = 8); or 0.1 mg of E-17 beta given into the stroma of the ovary, immediately adjacent to the dominant follicle (intraovarian (io), n = 6). Local (iu and io) treatments were given via a transvaginal ultrasound-guided needle injection. Treatment with 5 mg of E-17 beta im resulted in suppression of the dominant follicle of the first follicular wave and early emergence of the second follicular wave (P < 0.05). Diameter profiles of the dominant follicle in heifers treated with 0.1 mg im or 0.1 mg iu differed from those of control heifers on Day 5, whereas diameter profiles of the dominant follicle in heifers treated with 0.1 mg io did not differ from the controls. Daily changes in diameter of the dominant follicle did not differ among the three groups treated with 0.1 mg of E-17 beta (im, iu and io). Hourly changes in circulating concentrations of FSH and LH were not detected following estradiol treatment either before or after the results were combined for all estradiol-treated groups. Results are supportive of the hypothesis that the suppressive effect of estradiol in cattle is exerted indirectly through a systemic route rather than directly at the ovary. Although low plasma concentrations of FSH and LH were not detected, systemic treatments with high E-17 beta dosages resulted in follicular suppression whereas local treatments with subminimal dosages, within the ovary bearing the dominant follicle, were without effect. (C) 2000 Elsevier Science B.V. All rights reserved.