Electron transfer reaction of glucose oxidase hybrids modified with phenothiazine via poly(ethylene oxide) spacer on acidic amino acid residues

被引：7

作者：

Aoki, S ^{[1
]}

Ishii, K ^{[1
]}

Ueki, T ^{[1
]}

Ban, K ^{[1
]}

Imabayashi, S ^{[1
]}

Watanabe, M ^{[1
]}

机构：

[1] Yokohama Natl Univ, Fac Engn, Dept Chem & Biotechnol, Yokohama, Kanagawa 2408501, Japan

来源：

CHEMISTRY LETTERS | 2002年 / 02期

关键词：

D O I：

10.1246/cl.2002.256

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

GOx hybrids [GOx-(PT-PEO-NH2)] are prepared by covalently bonding phenothiazine (PT)-labeled poly(ethylene oxide) (PEO) oligomers having an amino end group, PT-PEO-NH2, to acidic amino acid residues on the enzyme surface, The rate constant for the mediated FADH/FADH(2) oxidation calculated from the catalytic current under substrate-saturated conditions ranges from 1.7 to 388 s(-1), and the largest value is obtained for GOx hybrids with PT-PEO of molecular weight 3000. Surprisingly effective electron transfer from FADH/FADH(2) to PT+ is achieved in the GOx-(PT-PEO-NH2) hybrids due to the PT modification to aspartic or glutamic acid residues, many of which are located close to the FAD center.

引用

页码：256 / 257

页数：2

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