TiO2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes

被引：122

作者：

Wang, Ronghui ^{[1
]}

Ruan, Chuanmin ^{[1
]}

Kanayeva, Damira ^{[2
]}

Lassiter, Kentu ^{[3
]}

Li, Yanbin ^{[1
,2
,3
]}

机构：

[1] Univ Arkansas, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA

[2] Univ Arkansas, Cell & Mol Biol Program, Fayetteville, AR 72701 USA

[3] Univ Arkansas, Ctr Excellence Poultry Sci, Fayetteville, AR 72701 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 09期

关键词：

D O I：

10.1021/nl080366q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel TiO2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10(2) cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.

引用

页码：2625 / 2631

页数：7

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