Pyrosequencing analysis of the Oral Microflora of healthy adults

被引：487

作者：

Keijser, B. J. F. ^{[3
]}

Zaura, E. ^{[1
,2
]}

Huse, S. M. ^{[4
]}

van der Vossen, J. M. B. M. ^{[3
]}

Schuren, F. H. J. ^{[3
]}

Montijn, R. C. ^{[3
]}

ten Cate, J. M. ^{[1
,2
]}

Crielaard, W. ^{[1
,2
]}

机构：

[1] Univ Amsterdam, Acad Ctr Dent Amsterdam, Dept Cariol Endodontol Pedodontol, NL-1066 EA Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, NL-1066 EA Amsterdam, Netherlands

[3] Microbial Genom Grp, Business Unit Food & Biotechnol Innovat, TNO Qual Life, Zeist, Netherlands

[4] Josephine Bay Paul Ctr, Marine Biol Lab, Woods Hole, MA USA

来源：

JOURNAL OF DENTAL RESEARCH | 2008年 / 87卷 / 11期

基金：

美国国家卫生研究院;

关键词：

pyrosequencing; microflora; plaque; saliva; diversity;

D O I：

10.1177/154405910808701104

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A good definition of commensal microflora and an understanding of its relation to health are essential in preventing and combating disease. We hypothesized that the species richness of human oral microflora is underestimated. Saliva and supragingival plaque were sampled from 71 and 98 healthy adults, respectively. Amplicons from the V6 hypervariable region of the small-subunit ribosomal RNA gene were generated by PCR, pooled into saliva and plaque pools, and sequenced by means of the Genome Sequencer 20 system at 454 Life Sciences. Data were evaluated by taxonomic and rarefaction analyses. The 197,600 sequences generated yielded about 29,000 unique sequences, representing 22 taxonomic phyla. Grouping the sequences in operational taxonomic units (6%) yielded 3621 and 6888 species-level phylotypes in saliva and plaque, respectively. This work gives a radically new insight into the diversity of human oral microflora, which, with an estimated number of 19,000 phylotypes, is considerably higher than previously reported. Abbreviations: GS-20, Genome Sequencer 20; OTU, Operational Taxonomic Unit; PCR, polymerase chain-reaction; 16S rDNA, smallsubunit (16S) ribosomal deoxyribonucleic acid; UV, ultraviolet.

引用

页码：1016 / 1020

页数：5

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