Sweet Substitute:: A software tool for in silico fragmentation of peptide-linked N-glycans

被引：15

作者：

Clerens, S

Van den Ende, W

Verhaert, P

Geenen, L

Arckens, L

机构：

[1] Katholieke Univ Leuven, Lab Neuroplast & Neuroproteom, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Dev Biol Lab, B-3001 Heverlee, Belgium

[3] NV Organon, Proteom Grp, Target Discovery Unit, Oss, Netherlands

来源：

PROTEOMICS | 2004年 / 4卷 / 03期

关键词：

glycosylation; mass spectrometry; N-linked glycane; tandem mass spectrometry;

D O I：

10.1002/pmic.200300572

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A software tool, Sweet Substitute, is described, which assists tandem mass spectrometry (MS/MS)-based glycosylation characterization from within a tryptic digest. The algorithm creates a virtual nanoelectrospray-quadrupole time-of-flight style-MS/MS spectrum of any user-defined N-linked glycan structure. An empirical peak height modeling routine is implemented in the program. By comparing the theoretical MS/MS data with the deconvoluted and deisotoped experimental MS/MS data, the user is able to quickly assess whether a proposed candidate oligosaccharide structure is a plausible one.

引用

页码：629 / 632

页数：4