The spliceosome: No assembly required?

被引：49

作者：

Nilsen, TW ^{[1
]}

机构：

[1] Case Western Reserve Univ, Sch Med, Ctr RNA Mol Biol, Cleveland, OH 44106 USA

来源：

MOLECULAR CELL | 2002年 / 9卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(02)00430-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this issue of Molecular Cell, Stevens et al. purify a large particle from yeast extracts that contains all five of the U snRNPs required for pre-mRNA splicing. The existence of this "penta-snRNP" suggests the provocative possibility that spliceosome assembly does not depend upon a pre-mRNA substrate.

引用

页码：8 / 9

页数：2

共 10 条

[1]

DAS R, 2000, MOL CELL, V6, P194

[2] Pre-mRNA splicing in the new millennium [J].

Hastings, ML ;

Krainer, AR .

CURRENT OPINION IN CELL BIOLOGY, 2001, 13 (03) :302-309

[3] Characterization of U4 and U6 interactions with the 5′ splice site using a S-cerevisiae in vitro trans-splicing system [J].

Johnson, TL ;

Abelson, J .

GENES & DEVELOPMENT, 2001, 15 (15) :1957-1970

[4] ASSOCIATION OF U2, U4, U5, AND U6 SMALL NUCLEAR RIBONUCLEOPROTEINS IN A SPLICEOSOME-TYPE COMPLEX IN ABSENCE OF PRECURSOR RNA [J].

KONARSKA, MM ;

SHARP, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5459-5462

[5] Functional recognition of the 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly [J].

Maroney, PA ;

Romfo, CM ;

Nilsen, TW .

MOLECULAR CELL, 2000, 6 (02) :317-328

[6]

MOORE MJ, 1993, RNA WORLD, P303

[7]

Nilsen TW, 1998, COLD SPRING HARBOR M, P279

[8] Mechanical devices of the spliceosome: Motors, clocks, springs, and things [J].

Staley, JP ;

Guthrie, C .

CELL, 1998, 92 (03) :315-326

[9] Composition and functional characterization of the yeast spliceosomal Penta-snRNP [J].

Stevens, SW ;

Ryan, DE ;

Ge, HY ;

Moore, RE ;

Young, MK ;

Lee, TD ;

Abelson, J .

MOLECULAR CELL, 2002, 9 (01) :31-44

[10] Spliceosomal UsnRNP biogenesis, structure and function [J].

Will, CL ;

Lührmann, R .

CURRENT OPINION IN CELL BIOLOGY, 2001, 13 (03) :290-301

← 1 →