Ultrasensitive detection of proteins by amplification of affinity aptamers

被引：109

作者：

Zhang, HQ ^{[1
]}

Wang, ZW ^{[1
]}

Li, XF ^{[1
]}

Le, XC ^{[1
]}

机构：

[1] Univ Alberta, Dept Publ Hlth Sci, Edmonton, AB T6G 2G3, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 10期

关键词：

aptamers; DNA structures; polymerase chain reaction; protein detection; protein structures;

D O I：

10.1002/anie.200503345

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Superdetective: The detection of 180 molecules of a protein was achieved by amplification of DNA aptamers that bind to the target protein. The protein-aptamer complex is separated from the unbound aptamer by capillary electro-phoresis (CE). The complex fraction is collected and the aptamer amplified by the polymerase chain reaction (PCR). Such amplification dramatically improves the sensitivity of the detection of proteins. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1576 / 1580

页数：5

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