Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I real time RT-PCR

被引：174

作者：

Vandesompele, J ^{[1
]}

De Paepe, A ^{[1
]}

Speleman, F ^{[1
]}

机构：

[1] State Univ Ghent Hosp, Ctr Med Genet, B-9000 Ghent, Belgium

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 303卷 / 01期

关键词：

D O I：

10.1006/abio.2001.5564

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：95 / 98

页数：4

共 8 条

[1] The elimination of primer-dimer accumulation in PCR
Brownie, J
Shawcross, S
Theaker, J
Whitcombe, D
Ferrie, R
Newton, C
Little, S
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (16) : 3235 - 3241
[2] Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
Bustin, SA
[J]. JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2000, 25 (02) : 169 - 193
[3] The DNA sequence of human chromosome 22
Dunham, I
Shimizu, N
Roe, BA
Chissoe, S
Dunham, I
Hunt, AR
Collins, JE
Bruskiewich, R
Beare, DM
Clamp, M
Smink, LJ
Ainscough, R
Almeida, JP
Babbage, A
Bagguley, C
Balley, J
Barlow, K
Bates, KN
Beasley, O
Bird, CP
Blakey, S
Bridgeman, AM
Buck, D
Burgess, J
Burrill, WD
Burton, J
Carder, C
Carter, NP
Chen, Y
Clark, G
Clegg, SM
Cobley, V
Cole, CG
Collier, RE
Connor, RE
Conroy, D
Corby, N
Coville, GJ
Cox, AV
Davis, J
Dawson, E
Dhami, PD
Dockree, C
Dodsworth, SJ
Durbin, RM
Ellington, A
Evans, KL
Fey, JM
Fleming, K
French, L
[J]. NATURE, 1999, 402 (6761) : 489 - 495
[4] The DNA sequence of human chromosome 21
Hattori, M
Fujiyama, A
Taylor, TD
Watanabe, H
Yada, T
Park, HS
Toyoda, A
Ishii, K
Totoki, Y
Choi, DK
Soeda, E
Ohki, M
Takagi, T
Sakaki, Y
Taudien, S
Blechschmidt, K
Polley, A
Menzel, U
Delabar, J
Kumpf, K
Lehmann, R
Patterson, D
Reichwald, K
Rump, A
Schillhabel, M
Schudy, A
Zimmermann, W
Rosenthal, A
Kudoh, J
Shibuya, K
Kawasaki, K
Asakawa, S
Shintani, A
Sasaki, T
Nagamine, K
Mitsuyama, S
Antonarakis, SE
Minoshima, S
Shimizu, N
Nordsiek, G
Hornischer, K
Brandt, P
Scharfe, M
Schön, O
Desario, A
Reichelt, J
Kauer, G
Blöcker, H
Ramser, J
Beck, A
[J]. NATURE, 2000, 405 (6784) : 311 - 319
[5] Real time quantitative PCR
Heid, CA
Stevens, J
Livak, KJ
Williams, PM
[J]. GENOME RESEARCH, 1996, 6 (10): : 986 - 994
[6] KINETIC PCR ANALYSIS - REAL-TIME MONITORING OF DNA AMPLIFICATION REACTIONS
HIGUCHI, R
FOCKLER, C
DOLLINGER, G
WATSON, R
[J]. BIO-TECHNOLOGY, 1993, 11 (09): : 1026 - 1030
[7] Advances in quantitative PCR technology:: 5′ nuclease assays
Lie, YS
Petropoulos, CJ
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (01) : 43 - 48
[8] Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
Ririe, KM
Rasmussen, RP
Wittwer, CT
[J]. ANALYTICAL BIOCHEMISTRY, 1997, 245 (02) : 154 - 160

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