Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly(HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres

被引:57
作者
Bílková, Z
Slováková, M
Lycka, A
Horák, D
Lenfeld, J
Turková, J
Churácek, J
机构
[1] Univ Pardubice, Dept Biol & Biochem Sci, Pardubice 53210, Czech Republic
[2] Univ Pardubice, Dept Analyt Chem, Pardubice 53210, Czech Republic
[3] Res Inst Organ Synth, Pardubice 53218, Rybitvi, Czech Republic
[4] Acad Sci Czech Republ, Inst Macromol Chem, Prague 16206 6, Czech Republic
[5] Acad Sci Czech Republ, Inst Organ Chem & Biochem, Prague 16610 6, Czech Republic
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 770卷 / 1-2期
关键词
oriented immobilization; magnetic sorbents; enzymes; galactose oxidase;
D O I
10.1016/S0378-4347(01)00439-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
order to obtain an active and stable oxidation reactor for daily use in biochemical laboratory we decided to immobilize galactose oxidase orientedly through a carbohydrate chain to the magnetic carriers. We used hydrazide derivatives of non-magnetic and magnetic bead cellulose and of magnetic and non-magnetic poly(HEMA-co-EDMA) microspheres. Activation of the enzyme molecules was done by sodium periodate in the presence of supplements (fucose, CuSO4, catalase). Orientedly immobilized galactose oxidase presents high storage stability and lower susceptibility to inappropriate microenvironmental conditions. Reactor reactivated by three pulses of D-galactose retained practically 100% of its native activity after 6 months. The positive properties of both magnetic carriers were entirely confirmed. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:25 / 34
页数:10
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