Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed

被引：360

作者：

Gardel, Margaret L. ^{[1
]}

Sabass, Benedikt ^{[2
,3
,4
]}

Ji, Lin ^{[5
]}

Danuser, Gaudenz ^{[5
]}

Schwarz, Ulrich S. ^{[2
,3
,4
]}

Waterman, Clare M. ^{[6
]}

机构：

[1] Univ Chicago, Dept Phys, Chicago, IL 60637 USA

[2] Univ Heidelberg, BIOQUANT, D-69120 Heidelberg, Germany

[3] Univ Karlsruhe, Inst Zool, D-76131 Karlsruhe, Germany

[4] Karlsruhe Inst Technol, D-76131 Karlsruhe, Germany

[5] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[6] NHLBI, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CELL BIOLOGY | 2008年 / 183卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1083/jcb.200810060

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

How focal adhesions (FAs) convert retrograde filamentous actin (F-actin) flow into traction stress on the extracellular matrix to drive cell migration is unknown. Using combined traction force and fluorescent speckle microscopy, we observed a robust biphasic relationship between F-actin speed and traction force. F-actin speed is inversely related to traction stress near the cell edge where FAs are formed and F-actin motion is rapid. In contrast, larger FAs where the F-actin speed is low are marked by a direct relationship between F-actin speed and traction stress. We found that the biphasic switch is determined by a threshold F-actin speed of 8-10 nm/s, independent of changes in FA protein density, age, stress magnitude, assembly/disassembly status, or subcellular position induced by pleiotropic perturbations to Rho family guanosine triphosphatase signaling and myosin II activity. Thus, F-actin speed is a fundamental regulator of traction force at FAs during cell migration.

引用

页码：999 / 1005

页数：7

共 26 条

[1] Comparative Dynamics of Retrograde Actin Flow and Focal Adhesions: Formation of Nascent Adhesions Triggers Transition from Fast to Slow Flow [J].