The Cmr complex: an RNA-guided endoribonuclease

被引：4

作者：

Bailey, Scott ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biochem & Mol Biol, Bloomberg Sch Publ Hlth, Baltimore, MD 21205 USA

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2013年 / 41卷

关键词：

clustered regularly interspaced short palindromic repeats (CRISPR); Cmr; repeat-associated mysterious protein (RAMP); RNA interference; metal ion; nuclease; CRISPR-CAS SYSTEMS; SILENCING COMPLEX; CRYSTAL-STRUCTURE; IMMUNE-SYSTEM; INTERFERENCE; MECHANISM; PROTEIN; SULFOLOBUS; NUCLEASE; DNA;

D O I：

10.1042/BST20130216

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system protects prokaryotes from infection by viruses and other potential genome invaders. This system represents an inheritable and adaptable immune system that is mediated by large ribonucleoprotein complexes, the CRISPR-Cas effector complexes. The Cmr complex is unique among CRISPR-Cas effector complexes in that it destroys invading RNA and not DNA. To date, the Cmr complexes from two species have been characterized in vitro and, strikingly, they degrade RNA via distinct mechanisms. The possible in vivo targets, as well as our current knowledge of the Cmr complex, is reviewed in the present paper.

引用

页码：1464 / 1467

页数：4

共 26 条

[1] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[2] Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference [J].

Beloglazova, Natalia ;

Petit, Pierre ;

Flick, Robert ;

Brown, Greg ;

Savchenko, Alexei ;

Yakunin, Alexander F. .

EMBO JOURNAL, 2011, 30 (22) :4616-4627

[3] Identification of Novel Positive-Strand RNA Viruses by Metagenomic Analysis of Archaea-Dominated Yellowstone Hot Springs [J].

Bolduc, Benjamin ;

Shaughnessy, Daniel P. ;

Wolf, Yuri I. ;

Koonin, Eugene V. ;

Roberto, Francisco F. ;

Young, Mark .

JOURNAL OF VIROLOGY, 2012, 86 (10) :5562-5573

[4] Structure of the Cmr2 Subunit of the CRISPR-Cas RNA Silencing Complex [J].

Cocozaki, Alexis I. ;

Ramie, Nancy F. ;

Shao, Yaming ;

Hale, Caryn R. ;

Terns, Rebecca M. ;

Terns, Michael P. ;

Li, Hong .

STRUCTURE, 2012, 20 (03) :545-553

[5] A novel interference mechanism by a type IIIB CRISPR-Cmr module in Sulfolobus [J].

Deng, Ling ;

Garrett, Roger A. ;

Shah, Shiraz A. ;

Peng, Xu ;

She, Qunxin .

MOLECULAR MICROBIOLOGY, 2013, 87 (05) :1088-1099

[6] Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus [J].

Deng, Ling ;

Kenchappa, Chandra S. ;

Peng, Xu ;

She, Qunxin ;

Garrett, Roger A. .

NUCLEIC ACIDS RESEARCH, 2012, 40 (06) :2470-2480

[7] CRISPR-based immune systems of the Sulfolobales: complexity and diversity [J].

Garrett, Roger A. ;

Shah, Shiraz A. ;

Vestergaard, Gisle ;

Deng, Ling ;

Gudbergsdottir, Ley ;

Kenchappa, Chandra S. ;

Erdmann, Susanne ;

She, Qunxin .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2011, 39 :51-57

[8] Prokaryotic silencing (psi) RNAs in Pyrococcus furiosus [J].

Hale, Caryn ;

Kleppe, Kyle ;

Terns, Rebecca M. ;

Terns, Michael P. .

RNA, 2008, 14 (12) :2572-2579

[9] Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs [J].

Hale, Caryn R. ;

Majumdar, Sonali ;

Elmore, Joshua ;

Pfister, Neil ;

Compton, Mark ;

Olson, Sara ;

Resch, Alissa M. ;

Glover, Claiborne V. C., III ;

Graveley, Brenton R. ;

Terns, Rebecca M. ;

Terns, Michael P. .

MOLECULAR CELL, 2012, 45 (03) :292-302

[10] RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex [J].

Hale, Caryn R. ;

Zhao, Peng ;

Olson, Sara ;

Duff, Michael O. ;

Graveley, Brenton R. ;

Wells, Lance ;

Terns, Rebecca M. ;

Terns, Michael P. .

CELL, 2009, 139 (05) :945-956

← 1 2 3 →