Ku is associated with the telomere in mammals

被引:264
作者
Hsu, HL
Gilley, D
Blackburn, EH
Chen, DJ [1 ]
机构
[1] Univ Calif Berkeley, Div Life Sci, Berkeley, CA 94720 USA
[2] Univ Calif Los Alamos Natl Lab, Div Life Sci, Los Alamos, NM 87545 USA
[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA
关键词
D O I
10.1073/pnas.96.22.12454
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Telomeres are specialized DNA/protein complexes that comprise the ends of eukaryotic chromosomes. The highly expressed Ku heterodimer, composed of 70 and 80 K-d subunits (Ku70 and Ku80), is the high-affinity DNA binding component of the DNA-dependent protein kinase. Ku is critical for nonhomologous DNA double-stranded break repair and site-specific recombination of V(D)J gene segments. Ku also plays an important role in telomere maintenance in yeast, Herein, we report, using an in vivo crosslinking method, that human and hamster telomeric DNAs specifically coimmuno-precipitate with human Ku80 after crosslinking. Localization of Ku to the telomere does not depend on the DNA-dependent protein kinase catalytic component, These findings suggest a direct link between Ku and the telomere in mammalian cells.
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页码:12454 / 12458
页数:5
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