Integration of rapid DNA hybridization and capillary zone electrophoresis using bidirectional isotachophoresis

被引：34

作者：

Bahga, Supreet S. ^{[1
]}

Han, Crystal M. ^{[1
]}

Santiago, Juan G. ^{[1
]}

机构：

[1] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

来源：

ANALYST | 2013年 / 138卷 / 01期

关键词：

D O I：

10.1039/c2an36249j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a method for rapid, sequence-specific detection of multiple DNA fragments by integrating isotachophoresis (ITP) based DNA hybridization and capillary zone electrophoresis (CZE) using bidirectional ITP. Our method leverages the high preconcentration ability of ITP to accelerate slow, second-order DNA hybridization kinetics, and the high resolving power of CZE to separate and identify reaction products. We demonstrate the speed and sensitivity of our assay by detecting 5 pM, 39 nt ssDNA target within 3 min, using a molecular beacon probe. We also demonstrate the feasibility of our assay for multiplexed detection of multiple-length ssDNA targets by simultaneously detecting 39 and 90 nt ssDNA targets.

引用

页码：87 / 90

页数：4

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