Peptide- and biotin-oligonucleotide-pyrrole conjugates for electrochemical addressing on silicon chip

The syntheses of pyrrole-oligonucleotide-peptide conjugates and pyrroleoligonucleotide-biotin conjugates were described. The oligonucleotide moiety acted as an <<active linker>> which allowed the easy purification and quantitation of the conjugates and in turn controlled the grafting. The peptide conjugates were immobilised on a silicon array and their immunoreactivity was tested using biotinylated antibodies and a phycoerythrin-streptavidin staining. The biotin conjugate provided a fluorescence scale.