On-line coupling of liquid chromatography at critical conditions with electrospray ionization tandem mass spectrometry for the characterization of a nitroxide-mediated poly(ethylene oxide)/polystyrene block copolymer

被引：30

作者：

Girod, Marion ^{[1
,2
,3
]}

Phan, Trang N. T. ^{[1
,2
,3
]}

Charles, Laurence ^{[1
,2
,3
]}

机构：

[1] Univ Aix Marseille 1, CNRS, Lab Chim Prov, UMR 6264, F-13397 Marseille 20, France

[2] Univ Aix Marseille 2, CNRS, Lab Chim Prov, UMR 6264, F-13397 Marseille 20, France

[3] Univ Aix Marseille 3, CNRS, Lab Chim Prov, UMR 6264, F-13397 Marseille 20, France

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2008年 / 22卷 / 23期

关键词：

D O I：

10.1002/rcm.3796

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coupling of liquid chromatography at critical conditions (LCCC) with on-line mass spectrometry (MS) detection was implemented via an electrospray ionization (ESI) interface, using a mobile phase containing the cationizing agent. Critical conditions established for poly(ethylene oxide) were used to characterize a poly(ethylene oxide)/polystyrene block copolymer (PEO-b-PS) in both MS and MS/MS modes. As co-oligomer molecules were successfully separated according to the PS block size, structural information could be reached from simplified MS spectra. The microstructure of this copolymer, synthesized by nitroxide-mediated polymerization, could further be unambiguously characterized in LCCC/ESI-MS/MS experiments since the PS block size could be reached by both the co-oligomer chromatographic behavior and its MS/MS pattern. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：3767 / 3775

页数：9

共 63 条

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