Deacetylation of the herpes simplex virus type 1 latency-associated transcript (LAT) enhancer and a decrease in LAT abundance precede an increase in ICP0 transcriptional permissiveness at early times postexplant

被引:67
作者
Amelio, AL [1 ]
Giordani, NV [1 ]
Kubat, NJ [1 ]
O'Neil, JE [1 ]
Bloom, DC [1 ]
机构
[1] Univ Florida, Coll Med, Dept Mol Genet & Micorbiol, Gainesville, FL 32610 USA
关键词
D O I
10.1128/JVI.80.4.2063-2068.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Only the latency-associated transcript (LAT) of the herpes simplex virus type 1(HSV-1) genome is transcribed during latency, while the lytic genes are suppressed, possibly by LAT antisense mechanisms and/or chromatin modifications. In the present study, latently infected dorsal root ganglia were explanted to assess both relative levels of LAT and histone H3 (K9, K14) acetylation of the LAT locus and ICPO promoter at early times postexplant. We observed that a decrease in both LAT enhancer histone H3 (K9, K14) acetylation and LAT RNA abundance occurs prior to an increase in acetylation, or transcriptional permissiveness, at the ICPO promoter.
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收藏
页码:2063 / 2068
页数:6
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