The effect orthosilicic acid on collagen type I, alkaline phosphatase and osteocalcin mRNA expression in human bone-derived osteoblasts in vitro

被引：5

作者：

Arumugam, MQ

Ireland, DC

Brooks, RA

Rushton, N

Bonfield, W

机构：

[1] Univ Cambridge, Dept Mat Sci & Met, Cambridge Ctr Med Mat, Cambridge CB2 3QZ, England

[2] Univ Cambridge, Dept Med, Bone Med Grp, Cambridge CB2 2QQ, England

[3] Univ Cambridge, Orthopaed Res Unit, Dept Surg, Addenbrookes Hosp, Cambridge CB2 2QQ, England

来源：

BIOCERAMICS 18, PTS 1 AND 2 | 2006年 / 309-311卷

关键词：

silicon; orthosilicic acid; human osteoblast cells; reverse transcriptase-polymerase chain reaction; collagen type I; alkaline phosphatase; osteocalcin;

D O I：

10.4028/www.scientific.net/KEM.309-311.121

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 [材料科学与工程]; 080502 [材料学];

摘要：

The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10 mu M) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type 1, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10 mu M orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased.

引用

页码：121 / 124

页数：4

共 11 条

[1]

Orthosilicic acid increases collagen type I mRNA expression in human bone-derived osteoblasts in vitro [J].