Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1

被引：235

作者：

Trotman, LC

Mosberger, N

Fornerod, M

Stidwill, RP

Greber, UF

机构：

[1] Univ Zurich, Inst Zool, CH-8057 Zurich, Switzerland

[2] Netherlands Canc Inst, NL-1066 CX Amsterdam, Netherlands

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 12期

关键词：

D O I：

10.1038/ncb1201-1092

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Adenovirus type 2 (Ad2) imports its DNA genome through the nuclear pore complex (NPC) of cells in interphase for viral production. Here we identify the NPC-filament protein CAN/Nup214 as a docking site for incoming Ad2 capsids. Binding to CAN is independent of cytosolic factors. Capsids disassemble at NPCs to free their DNA for import. This process requires binding of nuclear histone HI to the stably docked capsids and involves Hl-import factors, restricting this irreversible process to the proximity of the nucleus. Our results provide a molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import.

引用

页码：1092 / 1100

页数：9

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