Bone tissue engineering based on bead-cell sheets composed of calcium phosphate beads and bone marrow cells

被引：17

作者：

Furukawa, KS

Miyauchi, S

Suzuki, D

Umezu, Y

Shinjo, T

Ushida, T

Eguchi, M

Tateishi, T

机构：

[1] Univ Tokyo, Dept Mech Engn, Grad Sch Engn,Bunkyo Ku, Biomed Engn Ushida & Furukawa Lab, Tokyo 1138656, Japan

[2] Natl Inst Mat Sci, Ctr Biomat, Ibaraki, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2004年 / 24卷 / 03期

关键词：

bone tissue engineering; sheet; beads; bone marrow cells;

D O I：

10.1016/j.msec.2003.11.010

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We developed a new tissue-engineered bone graft model composed of bovine bone marrow stromal cells (BMSCs), and beta tricalcium phosphate (beta TCP) porous beads. Such cell-bead sheets were prepared by means of cultivation of BMSCs with the beads in alpha-MEM culture medium supplemented with 10% fetal bovine serum. The shape of the sheets was possible to change depending on that of a plastic mold having a circle or quadrangle shape. Addition of poundage on the overlaid cell-bead sheets induced the connection of these sheets with each other, and achievement of thicker implant formation. A nonwoven fabric sheet Of poly-L-lactic acid (PLLA) could also be inserted in between each cell-bead sheet. Consequently, bone implants having a size of 20 x 20 x 5 unit were successfully developed. This kind of techniques would be useful in thick implants for other tissue implants. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：437 / 440

页数：4

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