The identification of a new actin-binding region in p57

被引：11

作者：

Liu, CZ ^{[1
]}

Chen, Y ^{[1
]}

Sui, SF ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembranes, Beijing 100084, Peoples R China

来源：

CELL RESEARCH | 2006年 / 16卷 / 01期

关键词：

actin-binding protein; p57; actin cytoskeleton; leucine zipper;

D O I：

10.1038/sj.cr.7310014

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The actin-binding protein p57 is a member of mammalian coronin-like proteins. The roles of this protein in phagocytic processes conceivably depend on its interactions with F-actin. Two regions, p57(1-34) and p57(111-204), were previously reported to be actin-binding sites. In this study, we found that the C-terminal region of p57, p57(297-461), also possessed F-actin binding activity. Furthermore, the leucine zipper domain at the C-terminus of p57(297-461) was essential for this actin-binding activity. The F-actin cross-linking assay revealed that the region contained in p57(297-461) was sufficient to cross-link actin filaments. Our results strongly suggested that there was a new actin-binding region at the C-terminus of p57.

引用

页码：106 / 112

页数：7

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