Screening for abnormalities of chromosomes X, Y, and 18 and for diploidy in spermatozoa from infertile men participating in an in vitro fertilization-intracytoplasmic sperm injection program

被引：85

作者：

Aran, B

Blanco, J

Vidal, F

Vendrell, JM

Egozcue, S

Barri, PN

Egozcue, J

Veiga, A

机构：

[1] Inst Univ Dexeus, Dept Obstet & Gynecol, Servei Med Reprod, E-08017 Barcelona, Spain

[2] Univ Autonoma Barcelona, Fac Ciencies, Unitat Biol Cellular, Bellaterra, Spain

来源：

FERTILITY AND STERILITY | 1999年 / 72卷 / 04期

关键词：

fluorescence in situ hybridization; sperm aneuploidy; sperm diploidy; ICSI;

D O I：

10.1016/S0015-0282(99)00307-6

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To evaluate the frequency of disomy (for chromosomes X, Y, and 18) and of diploidy in the spermatozoa of infertile men undergoing intracytoplasmic sperm injection (ICSI). Design: Prospective analysis of sperm nuclei by fluorescence in situ hybridization (FISH). Setting: University-affiliate IVF-ICSI program. Patient(s): Semen samples from 19 patients participating in an IVF-ICSI program. Intervention(s): Semen samples were analyzed and prepared for FISH. Main Outcome Measure(s): Semen parameters were evaluated. The frequency of disomy for chromosomes X, Y, and 18 and the frequency of diploidy were analyzed by FISH. Result(s): A total of 9,373 spermatozoa from 19 infertile patients were analyzed and compared with spermatozoa from a control group of 5 healthy men. No differences in the frequency of disomy 18 were found, but statistically significant differences in the incidence of sex chromosome disomy and of diploidy were observed. Conclusion(s): The study of sperm nuclei by FISH is useful to improve genetic counseling in infertile patients selected for ICSI. (Fertil Steril(R) 1999;72:699-701. (C) 1999 by American Society for Reproductive Medicine.).

引用

页码：696 / 701

页数：6

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