C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells

被引：16

作者：

Baltus, B

Buitenhuis, M

van Dijk, TB

Vinson, C

Raaijmakers, JAM

Lammers, JWJ

Koenderman, L

de Groot, RP

机构：

[1] Univ Utrecht Hosp, Dept Pulm Dis, NL-3584 CX Utrecht, Netherlands

[2] NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1999年 / 66卷 / 04期

关键词：

granulocytes; transcription regulation;

D O I：

10.1002/jlb.66.4.683

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34(+) cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.

引用

页码：683 / 688

页数：6

共 52 条

[1] A NUCLEAR FACTOR FOR IL-6 EXPRESSION (NF-IL6) IS A MEMBER OF A C/EBP FAMILY [J].