Staphylococcal enterotoxin C injection in combination with ascorbic acid promotes the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts in vitro

被引：28

作者：

Wan, Xiao-Chen ^{[2
]}

Liu, Cui-Ping ^{[2
]}

Li, Meng ^{[2
]}

Hong, Dun ^{[1
,2
]}

Li, Dong-Mei ^{[2
]}

Chen, Hai-Xiao ^{[1
]}

Li, Ji-Cheng ^{[2
]}

机构：

[1] Zhejiang Prov Taizhou Hosp, Dept Orthoped, Linhai 317000, Zhejiang, Peoples R China

[2] Zhejiang Univ, Inst Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 373卷 / 04期

关键词：

staphylococcal enterotoxin C injection; ascorbic acid; bone marrow-derived mesenchymal stem cells; differentiation; osteoblasts;

D O I：

10.1016/j.bbrc.2008.06.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Staphylococcal enterotoxin C injection is established as a clinical therapy for delayed healing or disunion of bone fractures. In the present study, the effects of staphylococcal enterotoxin C injection in combination with ascorbic acid (SEC-AA) on the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) and their influences on the mineralization of osteoblasts were investigated. SEC-AA treatment induced increased levels of alkaline phosphatase activity in MSCs and increased numbers of alizarin red-stained calcified nodules, indicating enhanced differentiation of MSCs into osteoblasts. The findings demonstrated that SEC-AA promoted the differentiation of MSCs into osteoblasts and accelerated the cytopoiesis of osteoblasts. Our data provide a cytological model for bone fracture therapy aimed at shortening the time required for healing and improving the clinical outcome, and also provide a theoretical basis for inducible differentiation of MSCs, mineralization of osteoblasts and reconstruction of bone tissues. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.

引用

页码：488 / 492

页数：5

共 17 条

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