Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples

被引：28

作者：

Affolabi, Dissou ^{[1
,3
]}

Odoun, Mathieu ^{[1
]}

Sanoussi, N'Dira ^{[1
]}

Martin, Anandi ^{[3
,4
]}

Palomino, Juan Carlos ^{[3
]}

Kestens, Luc ^{[2
]}

Anagonou, Severin ^{[1
]}

Portaels, Francoise ^{[3
]}

机构：

[1] Lab Reference Mycobacteries, Cotonou, Benin

[2] Inst Trop Med, Immunol Unit, B-2000 Antwerp, Belgium

[3] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium

[4] Med Sans Frontieres, F-75011 Paris, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 10期

关键词：

D O I：

10.1128/JCM.00083-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis, we applied the nitrate reductase assay (NRA) using a liquid medium directly to sputum samples. A total of 179 sputum samples were analyzed by the NRA, and results were compared to those obtained by the indirect proportion method (IPM) as a standard reference. Out of 144 specimens for which comparable results were available, only one discrepant result was obtained: MDR by NRA but susceptible by the IPM. In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries.

引用

页码：3243 / 3245

页数：3

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