Increased oxidative damage to DNA in a transgenic mouse model of Huntington's disease

被引：198

作者：

Bogdanov, MB

Andreassen, OA

Dedeoglu, A

Ferrante, RJ

Beal, MF

机构：

[1] Massachusetts Gen Hosp, Neurol Serv, Neurochem Lab, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Boston, MA USA

[3] Vet Adm Med Ctr, Ctr Geriatr Res Educ & Clin, Bedford, MA USA

[4] Boston Univ, Sch Med, Dept Neurol, Boston, MA 02118 USA

[5] Boston Univ, Sch Med, Dept Pathol, Boston, MA 02118 USA

[6] Boston Univ, Sch Med, Dept Psychiat, Boston, MA 02118 USA

[7] New York Presbyterian Hosp, New York, NY USA

来源：

JOURNAL OF NEUROCHEMISTRY | 2001年 / 79卷 / 06期

关键词：

free radicals; Huntington's disease; 8-hydroxy-2-deoxyguanosine; oxidative damage; microdialysis; transgenic;

D O I：

10.1046/j.1471-4159.2001.00689.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondrial dysfunction and oxidative damage may play a role in the pathogenesis of Huntington's disease (HD). We examined concentrations of 8-hydroxy-2-deoxyguanosine (OH(8)dG), a well-established marker of oxidative damage to DNA, in a transgenic mouse model of HD (R6/2). Increased concentrations of OH(8)dG were found in the urine, plasma and striatal microdialysates of the HD mice. Increased concentrations were also observed in isolated brain DNA at 12 and 14 weeks of age. Immunocytochemistry showed increased OH(8)dG staining in late stages of the illness. These results suggest that oxidative damage may play a role in the pathogenesis of neuronal degeneration in the R6/2 transgenic mouse model of HD.

引用

页码：1246 / 1249

页数：4

共 21 条

[1] No evidence for increased oxidative damage to lipids, proteins, or DNA in Huntington's disease
Alam, ZI
Halliwell, B
Jenner, P
[J]. JOURNAL OF NEUROCHEMISTRY, 2000, 75 (02) : 840 - 846
[2] Increased oxidative damage to DNA in ALS patients
Bogdanov, M
Brown, RH
Matson, W
Smart, R
Hayden, D
O'Donnell, H
Beal, MF
Cudkowicz, M
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 2000, 29 (07) : 652 - 658
[3] A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2 '-deoxyguanosine measurements in urine and other biologic matrices: A one-year evaluation of methods
Bogdanov, MB
Beal, MF
McCabe, DR
Griffin, RM
Matson, WR
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (5-6) : 647 - 666
[4] Oxidative damage and metabolic dysfunction in Huntington's disease: Selective vulnerability of the basal ganglia
Browne, SE
Bowling, AC
MacGarvey, U
Baik, MJ
Berger, SC
Muqit, MMK
Bird, ED
Beal, MF
[J]. ANNALS OF NEUROLOGY, 1997, 41 (05) : 646 - 653
[5] DISTRIBUTION OF PHOSPHATE-ACTIVATED GLUTAMINASE, SUCCINIC-DEHYDROGENASE, PYRUVATE-DEHYDROGENASE AND GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE IN POST-MORTEM BRAIN FROM HUNTINGTONS-DISEASE AND AGONAL CASES
BUTTERWORTH, J
YATES, CM
REYNOLDS, GP
[J]. JOURNAL OF THE NEUROLOGICAL SCIENCES, 1985, 67 (02) : 161 - 171
[6] Altered brain neurotransmitter receptors in transgenic mice expressing a portion of an abnormal human Huntington disease gene
Cha, JHJ
Kosinski, CM
Kerner, JA
Alsdorf, SA
Mangiarini, L
Davies, SW
Penney, JB
Bates, GP
Young, AB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) : 6480 - 6485
[7] CHENG KC, 1992, J BIOL CHEM, V267, P166
[8] DAMAGE TO THE DNA BASES IN MAMMALIAN CHROMATIN BY HYDROGEN-PEROXIDE IN THE PRESENCE OF FERRIC AND CUPRIC IONS
DIZDAROGLU, M
RAO, G
HALLIWELL, B
GAJEWSKI, E
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 285 (02) : 317 - 324
[9] Ferrante RJ, 1997, J NEUROCHEM, V69, P2064
[10] OXIDATIVE DNA-DAMAGE INDUCED BY SIMULTANEOUS GENERATION OF NITRIC-OXIDE AND SUPEROXIDE
INOUE, S
KAWANISHI, S
[J]. FEBS LETTERS, 1995, 371 (01) : 86 - 88

← 1 2 3 →