Feedback inhibition of epithelial Na+ channels in Xenopus oocytes does not require G0 or Gi2 proteins

被引:12
作者
Hübner, M
Schreiber, R
Boucherot, A
Sanchez-Perez, A
Poronnik, P
Cook, DI
Kunzelmann, K [1 ]
机构
[1] Univ Sydney, Dept Physiol F13, Sydney, NSW 2006, Australia
[2] Univ Freiburg, Inst Physiol, D-79104 Freiburg, Germany
关键词
Na+ feedback; epithelial Na+ channel; Xenopus oocyte; G protein; G(i2); G(0);
D O I
10.1016/S0014-5793(99)01291-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Regulation of amiloride-sensitive epithelial Na+ channels (ENaC) is a prerequisite for coordination of electrolyte transport in epithelia, Downregulation of Na+ conductance occurs when the intracellular Na+ concentration is increased during reabsorption of electrolytes, known as feedback inhibition, Recent studies have demonstrated the involvement of alpha G(0) and alpha G(i2) proteins in the feedback control of ENaC in mouse salivary duct cells, In this report, we demonstrate that Na+ feedback inhibition is also present in Xenopus oocytes after expression of rat alpha,beta,gamma-ENaC. Interfering with intracellular alpha G(0) or alpha G(i2) Signaling by coexpression of either constitutively active alpha G(0)/alpha G(i2) or dominant negative alpha G(0)/alpha G(i2) and by coinjecting sense or antisense oligonucleotides for alpha G(0) had no impact on Na+ feedback, Moreover, no evidence for involvement of the intracellular G protein cascade was found in experiments in which a regulator of G protein signaling (RGS3) or beta-adrenergic receptor kinase (beta ARK) was coexpressed together with alpha,beta,gamma-ENaC. Although some experiments suggest the presence of an intracellular Na+ receptor, we may conclude that Na+ feedback in Xenopus oocytes is different from that described for salivary duct cells in that it does not require G protein signaling. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:443 / 447
页数:5
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