Synthesis and characterization of a chemically-bonded cholesteric stationary phase for high-performance liquid chromatography

被引：52

作者：

Delaurent, C

Tomao, V

Siouffi, AM

机构：

[1] Université d'Axis-Marseille III,UMR Synthèse et Catalyse, Faculté des Sciences et Techniques de St Jérome

来源：

CHROMATOGRAPHIA | 1997年 / 45卷 / 1期

关键词：

column liquid chromatography; cholesterol-bonded phase; spectroscopic and thermoanalytical characterization; chromatographic behaviour;

D O I：

10.1007/BF02505584

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bonded, cholesteric stationary phase for high performance liquid chromatography was prepared by reacting the cholesteryl chloroformate with the terminal amino group of a bonded aminopropyl silica from Merck. Infrared Fourier transform spectrometry; carbon-13 cross polarization magic angle spinning NMR and differential scanning calorimetry confirmed the success of the bonding reaction. Carbon elemental analysis indicated a high loading of the cholesteric moiety on the surface and the argon adsorption method was used to measure the specific surface of this new bonded stationary phase. Van't Hoff plots of both cholesteric phase and RP-18 confirm the different behaviour. Examples of selectivities are given.

引用

页码：355 / 363

页数：9

共 13 条

[1] PREPARATION AND CHROMATOGRAPHIC PROPERTIES OF SOME CHEMICALLY BONDED PHASES FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].