Binding of Zn(II), Cu(II), and Fe(II) ions to Alzheimer's Aβ peptide studied by fluorescence

被引：135

作者：

Garzon-Rodriguez, W ^{[1
]}

Yatsimirsky, AK

Glabe, CG

机构：

[1] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

[2] Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1999年 / 9卷 / 15期

关键词：

D O I：

10.1016/S0960-894X(99)00357-1

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Binding of Zn(II), Cu(II) and Fe(II) ions to A beta 1-40, A beta 1-42 and a single tryptophan mutant of A beta 1-40 in solution at pH 7.4 was studied by fluorescent titration. Job plots and fitting of titration curves revealed formation of 1:1 and 1:2 peptide-metal complexes. For dimeric peptides A beta 1-40 and A beta F4W the order of metal to peptide affinities is Fe < Cu > Zn, which is in agreement with the Irving-Williams series of complex stability. The affinity of A beta 1-42 for. Fe increases dramatically upon aggregation: K-D changes from ca. 100 to ca. 0.2 mu M. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：2243 / 2248

页数：6

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