Modular assembly of glycoproteins: Towards the synthesis of GlyCAM-1 by using expressed protein ligation

被引：83

作者：

Macmillan, D

Bertozzi, CR

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[2] Univ Calif Berkeley, Ctr New Direct Organ Synth, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Ctr New Direct Organ Synth, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 11期

关键词：

D O I：

10.1002/anie.200352673

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Put the two together: Physiologically relevant glycoproteins containing saccharides at predetermined sites, such as GlyCAM-1, can be generated from small synthetic and bacterially derived fragments by using the expressed protein ligation methodology. This technique was used for the synthesis of three semisynthetic GlyCAM-1 variants 1-3 (see scheme).

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页码：1355 / 1359

页数：5

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