DNA Microarray Analysis of the Heat Shock Transcriptome of the Obligate Intracytoplasmic Pathogen Rickettsia prowazekii

被引：20

作者：

Audia, Jonathon P. ^{[1
]}

Patton, Mary C. ^{[1
]}

Winkler, Herbert H. ^{[1
]}

机构：

[1] Univ S Alabama, Coll Med, Dept Microbiol & Immunol, Mol Biol Lab, Mobile, AL 36688 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 24期

关键词：

D O I：

10.1128/AEM.00896-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we present the first oligonucleotide DNA microarray analysis of global gene expression changes in the obligate intracytoplasmic pathogen Rickettsia prowazekii using temperature upshift as a model stress condition, and we describe a methodology for isolating highly purified rickettsial RNA. In toto, 23 transcripts were significantly increased by temperature upshift (>= 2.0-fold; P < 0.05), and no transcripts demonstrated reproducible decreases. Array results for three heat shock-inducible mRNAs were confirmed using quantitative reverse transcription-PCR.

引用

页码：7809 / 7812

页数：4

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