Biofilm production by isolates of Candida species recovered from nonneutropenic patients:: Comparison of bloodstream isolates with isolates from other sources

被引:277
作者
Shin, JH
Kee, SJ
Shin, MG
Kim, SH
Shin, DH
Lee, SK
Suh, SP
Ryang, DW
机构
[1] Chonnam Natl Univ, Sch Med, Dept Clin Pathol, Dong Ku, Gwangju 501757, South Korea
[2] Chonnam Natl Univ, Sch Med, Dept Internal Med, Dong Ku, Gwangju 501757, South Korea
[3] Seonam Univ, Coll Med, Dept Clin Pathol, Gwangju, South Korea
关键词
D O I
10.1128/JCM.40.4.1244-1248.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Biofilm production has been implicated as a potential virulence factor of some Candida species responsible for catheter-related fungemia in patients receiving parenteral nutrition. We therefore compared clinical bloodstream isolates representing seven different Candida species to each other and to those from other anatomical sites for the capacity to form biofilms in glucose-containing medium. Potential associations between the capacity to form biofilms and the clinical characteristics of fungemia were also analyzed. Isolates included the following from nonneutropenic patients: 101 bloodstream isolates (35 C parapsilosis, 30 C albicans, 18 C tropicalis, 8 C. glabrata, and 10 other Candida species isolates) and 259 clinical isolates from other body sites (116 C albicans, 53 C glabrata, 43 C. tropicalis, 17 C. parapsilosis, and 30 other Candida species isolates). Organisms were grown in Sabouraud dextrose broth (SDB) containing a final concentration of 8% glucose to induce biofilm formation, as published previously. Biofilm production was determined by both visual and spectrophotometric methods. In this medium, biofilm production by C albicans isolates was significantly less frequent (8%) than that by non-C albicans Candida species (61%; P < 0.0001). The overall proportion of non-C albicans Candida species isolates from the blood that produced biofilms was significantly higher than that of non-C. albicans Candida isolates obtained from other sites (79% versus 52%; P = 0.0001). Bloodstream isolates of C. parapsilosis alone were significantly more likely to be biofilm positive than were C. parapsilosis isolates from other sites (86% versus 47%; P = 0.0032). Non-C. albicans Candida species, including C. parapsilosis, were more likely to be biofilm positive if isolates were derived from patients whose candidemia was central venous catheter (CVC) related (95%; P < 0.0001) and was associated with the use of total parenteral nutrition (TPN) (94%; P < 0.005). These data suggest that the capacity of Candida species isolates to produce biofilms in vitro in glucose-containing SDB may be a reflection of the pathogenic potential of these isolates to cause CVC-related fungemia in patients receiving TPN.
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页码:1244 / 1248
页数:5
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